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Ovulation is the release of an egg from the ovary. The egg then enters the fallopian tube where it is ready to be fertilized. Conception of a child occurs when the male sperm successfully fertilizes the female egg. When a woman is about to ovulate, her body releases a large amount of a hormone called LH (luteinizing hormone). LH is always present in the urine but levels rise (soar) in the middle of the menstrual cycle, causing an egg to be released from the ovary.
The one-step ovulation test is an in vitrio immunochromatographic test for the qualitative and semiquantitative determination of human luteinizing hormone (LH) in urine to predict the ovulation phase in women. The test detects the sharp increase in the LH concentration in the urine, the so-called “LH surge” that precedes ovulation. Conception is more likely to take place within 36 hours of the LH surge.
Each sachet contains an immunochromatographic test. Each test contains a membrane with anti-LH antibodies and conjugates, in a stabilizing matrix containing proteins and sodium azide.
STORAGE AND STABILITY:
Store below 30C; do not freeze. LIMITATIONS: The one-step ovulation test is for in vitriotic diagnostic use only. Elevated Human Chorionic Gonadotropin (hCG) concentrations interfere with LH detection. Do not test specimens obtained from pregnant women and people with medical conditions that cause higher hCG levels. The one-step ovulation test is not designed to avoid conception. Since sperm can survive for 72 hours, you could still get pregnant if you had intercourse before the LH surge was detected.
WHEN TO START TESTING:
First of all, you need to establish the length of your menstrual cycle. This is a list of days from the first day of menstruation to the day before the start of the next period, count the first day of menstruation as day 1. Calculate how long your menstrual cycle was usually over the last months. After calculating the length of your menstrual cycle, refer to the table to determine which day of your menstrual cycle you should start testing.
Once you have identified the day to start the test, then begin to take urine daily.
1. Do not use first morning urine samples as LH is synthesized in the body early in the morning. It will appear in the urine only later in the day.
2. The best time to collect urine is between 10 am and 8 pm. Always do it at the same time that is most comfortable for you.
3. Collect urine at approximately the same time each day. Reduce the amount of liquid approximately 2 hours before collecting urine as the diluted urine sample can prevent detection of the test LH surge.
BEFORE YOU BEGIN:
1. Read the instructions carefully before starting the test.
2. Do not open the foil pouch until you are ready to test.
3. Make sure you have a watch or a wristwatch or a stopwatch available.
4. Allow urine samples and test kit to reach room temperature before testing (approximately 20 minutes).
1. Determine the day of the test.
2. Collect a urine sample in a clean, dry container.
3. To begin the test, open the sealed pouch and remove the strip. Do not remove the strip until you are ready to test.
4. With the arrows pointing down towards the urine, immerse the test strip in an upright (straight) position in the urine specimen for at least 10 seconds.
urine does not exceed the MAX (maximum) level marked by the band.
5. Remove the strip from the urine and place it on a clean, dry surface. If you want better results, read the results after 10 minutes. 6. Wait for the colored bands to appear. Depending on the LH concentration in the urine sample, positive results can be seen in no less than 40 seconds. However, a total reaction time of 30 minutes is required to confirm negative results.
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